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Addgene

CD3Zeta-GFP
(Plasmid #38298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD3Zeta
  • Alt name
    CD247
  • Alt name
    T-cell Receptor Zeta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    600
  • GenBank ID
    NM001113391
  • Entrez Gene
    Cd247 (a.k.a. 4930549J05Rik, A430104F18Rik, Cd3, Cd3-eta, Cd3-zeta, Cd3h, Cd3z, Cd3zeta, T3z, Tcrk, Tcrz)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD3Zeta-GFP was a gift from Matthew Krummel (Addgene plasmid # 38298 ; http://n2t.net/addgene:38298 ; RRID:Addgene_38298)

  • For your References section:

    Differential clustering of CD4 and CD3zeta during T cell recognition. Krummel MF, Sjaastad MD, Wulfing C, Davis MM. Science. 2000 Aug 25;289(5483):1349-52. 10.1126/science.289.5483.1349 PubMed 10958781
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