Myosin-IIA-GFP
(Plasmid #38297)

• Depositing Lab: Matthew Krummel
• Publication: Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 10608
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myosin II A
• Alt name: Myh9
• Alt name: MyoIIA heavy chain
• Alt name: Myhn1
• Species: M. musculus (mouse)
• Insert Size (bp): 5900
• GenBank ID: NM022410
• Entrez Gene: Myh9 (a.k.a. Fltn, Myhn-1, Myhn1, NMHC II-A, NMHCIIA, NMMHC-A, NMMHC-IIA, TU72.6)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry