pMK782 Septin6-GFP
(Plasmid #38296)

• Depositing Lab: Matthew Krummel
• Publication: Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6063
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Septin 6
• Alt name: Sept6
• Alt name: mKIAA0128
• Species: M. musculus (mouse)
• Insert Size (bp): 1363
• Mutation: 22 amino acid linker sequence (added by PCR)
• GenBank ID: NC_000086.7 BC010489-1
• Entrez Gene: Septin6 (a.k.a. 2810035H17Rik, C920001C06Rik, Sep, Sep6, Sept6, mKIAA0128)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry