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FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)
(Plasmid #38262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA 3.1 zeo-
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    β-arrestin2
  • Alt name
    Arrb2
  • Alt name
    BARRES
  • Species
    R. norvegicus (rat)
  • Mutation
    Arg 233, Arg237, and Lys251 converted to Glutamines, stop codon replaced with HindIII restriction site to create kinase domain fusion
  • GenBank ID
    NM_012911.1
  • Entrez Gene
    Arrb2 (a.k.a. BARRES)
  • Promoter CMV
  • Tags / Fusion Proteins
    • PIP5K Iα core kinase domain (residues 18-399) (C terminal on insert)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Point mutations described by Dr. Jim Keen.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K) was a gift from Robert Lefkowitz (Addgene plasmid # 38262 ; http://n2t.net/addgene:38262 ; RRID:Addgene_38262)

  • For your References section:

    Beta-arrestin scaffolding of phosphatidylinositol 4-phosphate 5-kinase Ialpha promotes agonist-stimulated sequestration of the beta2-adrenergic receptor. Nelson CD, Kovacs JJ, Nobles KN, Whalen EJ, Lefkowitz RJ. J Biol Chem. 2008 Jul 25;283(30):21093-101. Epub 2008 Jun 5. 10.1074/jbc.M800431200 PubMed 18534983
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