FLAG-β-arrestin2 RRK/Q
(Plasmid #38261)

• Depositing Lab: Robert Lefkowitz
• Publication: Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-101. Epub 2008 Jun 5.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: β-arrestin2
• Alt name: Arrb2
• Alt name: BARRES
• Species: R. norvegicus (rat)
• Mutation: Arg233, Arg237, and Lys251 converted to Glutamines
• GenBank ID: NM_012911.1
• Entrez Gene: Arrb2 (a.k.a. BARRES)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Point mutations described by Dr. Jim Keen.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry