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Addgene

β₂AR-mCFP
(Plasmid #38260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-mCFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    β₂AR
  • Alt name
    ADRB2
  • Alt name
    beta(2)AR
  • Species
    H. sapiens (human)
  • Mutation
    diglycine linker (replacing terminator codon)
  • GenBank ID
    NM_000024.5 NP_000015.1
  • Entrez Gene
    ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
  • Promoter CMV
  • Tags / Fusion Proteins
    • signal peptide sequence (MKTIIALSYIFCLVFA) (N terminal on insert)
    • Flag (N terminal on insert)
    • mCFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Lefkowitz Lab has confirmed this plasmid contains the human gene, and not the rat version described in the publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    β₂AR-mCFP was a gift from Robert Lefkowitz (Addgene plasmid # 38260 ; http://n2t.net/addgene:38260 ; RRID:Addgene_38260)

  • For your References section:

    G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer. Violin JD, Ren XR, Lefkowitz RJ. J Biol Chem. 2006 Jul 21;281(29):20577-88. Epub 2006 May 10. 10.1074/jbc.M513605200 PubMed 16687412
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