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pET22b-BirA-CPDSalI
(Plasmid #38258)

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Item Catalog # Description Quantity Price (USD)
Plasmid 38258 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene Plasmid #38251 (pET22b-CPDSalI)
  • Backbone manufacturer
    Matthew Bogyo laboratory
  • Backbone size w/o insert (bp) 6126
  • Total vector size (bp) 7106
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    birA
  • Alt name
    biotin ligase
  • Species
    E. coli
  • Insert Size (bp)
    980
  • Mutation
    cloned from pGEX4T1-BirA
  • GenBank ID
    NC_008563.1
  • Entrez Gene
    birA (a.k.a. APECO1_2494)
  • Promoter T7
  • Tags / Fusion Proteins
    • Vibrio cholerae MARTX toxin cysteine protease domain (CPD) (aa3440-3650) (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes untagged biotin ligase gene (birA) from E. coli fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used to purify biotin ligase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b-BirA-CPDSalI was a gift from Matthew Bogyo & Aimee Shen (Addgene plasmid # 38258 ; http://n2t.net/addgene:38258 ; RRID:Addgene_38258)

  • For your References section:

    Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag. Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, Garcia KC, Bogyo M. PLoS One. 2009 Dec 2;4(12):e8119. 10.1371/journal.pone.0008119 PubMed 19956581
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