pET22b-GFP-CPDSalI
(Plasmid #38257)

• Depositing Labs: Matthew Bogyo, Aimee Shen
• Publication: Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Backbone

• Vector backbone: Addgene Plasmid #38251 (pET22b-CPDSalI)
• Backbone manufacturer: Matthew Bogyo laboratory
• Backbone size w/o insert (bp): 6126
• Total vector size (bp): 6843
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 717
• Mutation: cloned from pEGFPN3 (Clonetech)
• Promoter: T7
• Tags / Fusion Proteins:
  • Vibrio cholerae MARTX toxin cysteine protease domain (CPD) (aa3440-3650) (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes untagged eGFP fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used as a positive control for protein purification