pLJM60-Auf1
(Plasmid #38242)

• Purpose: 3rd generation lentiviral vector
• Depositing Lab: David Sabatini
• Publication: Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.

Backbone

• Vector backbone: pLJM60
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AUF1
• Alt name: HNRNPD
• Species: H. sapiens (human)
• Insert Size (bp): 1067
• GenBank ID:
• Entrez Gene: HNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: ATGTCGGAGGAGCAGTTCGGCGGGGAC
• 3′ sequencing primer: TTAGTATGGTTTGTAGCTATTTTGATG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry