pTH734-CEN-minHIS3
(Plasmid #38227)

• Depositing Lab: Tobias von der Haar
• Publication: Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19.

Backbone

• Vector backbone: pCENBEVY-U
• Backbone size w/o insert (bp): 6614
• Total vector size (bp): 7300
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIS3
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 689
• Mutation: Every codon of the coding sequence has been replaced with the isoaccepting codon decoded by the least abundant tRNA.
• GenBank ID: NM_001183621
• Entrez Gene: HIS3 (a.k.a. YOR202W, HIS10, HIS8)
• Promoter: TDH3
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13r
• 3′ sequencing primer: M13f

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry