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Addgene

pTH729-minRLuc
(Plasmid #38225)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38225 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCENBEVY-U
  • Backbone size w/o insert (bp) 6614
  • Total vector size (bp) 7532
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla Luciferase
  • Alt name
    RLuc
  • Species
    Synthetic; Renilla reniformis
  • Insert Size (bp)
    941
  • Mutation
    Every codon of the Renilla coding sequence was exchanged for the isoaccepting codon with the lowest number of decoding tRNAs.
  • GenBank ID
    AF043450.1
  • Promoter TDH3 (=GPD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13r
  • 3′ sequencing primer M13f
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH729-minRLuc was a gift from Tobias von der Haar (Addgene plasmid # 38225 ; http://n2t.net/addgene:38225 ; RRID:Addgene_38225)

  • For your References section:

    Translation elongation can control translation initiation on eukaryotic mRNAs. Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T. EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19. 10.1002/embj.201385651 PubMed 24357599
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