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pTH738-CENBEVYslow
(Plasmid #38220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCENBEVY-U
  • Backbone size w/o insert (bp) 6614
  • Total vector size (bp) 7122
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCN4-based single uORF-containing 5'-UTR
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    500
  • Mutation
    The start codons of uORFs2, 3 and4 of the yeast Gcn4 leader sequence were mutated to non-start codons.
  • Promoter TDH3 (=GPD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13r
  • 3′ sequencing primer M13f
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The mutated GCN4 leder sequence originated from a plasmid kindly provided by Dr Leos Valasek (Academy of Sciences of the Czech Republic, Prague, Czech Republic). Grant CM, Miller PF, Hinnebusch AG (1994)Molecular & Cellular Proteomics 14:2616-2628.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH738-CENBEVYslow was a gift from Tobias von der Haar (Addgene plasmid # 38220 ; http://n2t.net/addgene:38220 ; RRID:Addgene_38220)

  • For your References section:

    Translation elongation can control translation initiation on eukaryotic mRNAs. Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T. EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19. 10.1002/embj.201385651 PubMed 24357599
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