Skip to main content
Addgene

pMXs-IP-EGFP-hAtg13
(Plasmid #38191)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMXs-IP
  • Backbone manufacturer
    Dr. Toshio Kitamura of the University of Tokyo
  • Backbone size w/o insert (bp) 5847
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy-related genes 13
  • Alt name
    Atg13
  • Alt name
    KIAA0652
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1560
  • GenBank ID
    AB014552
  • Entrez Gene
    ATG13 (a.k.a. KIAA0652, PARATARG8)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CAGCCCTCACTCCTTCTCTAG
  • 3′ sequencing primer GAGGAACTGCTTCCTTCACG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-IP-EGFP-hAtg13 was a gift from Noboru Mizushima (Addgene plasmid # 38191 ; http://n2t.net/addgene:38191 ; RRID:Addgene_38191)

  • For your References section:

    Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Hosokawa N, Hara T, Kaizuka T, Kishi C, Takamura A, Miura Y, Iemura S, Natsume T, Takehana K, Yamada N, Guan JL, Oshiro N, Mizushima N. Mol Biol Cell. 2009 Apr . 20(7):1981-91. 10.1091/mbc.e08-12-1248 PubMed 19211835
Related items:
Commonly requested with: