BCR/ABL P190 transgenic construct
(Plasmid #38185)

• Depositing Lab: Nora Heisterkamp
• Publication: Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

Backbone

• Vector backbone: pSK
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 13000
• Vector type: construct for making transgenic mice

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BCR/ABL P190
• Species: H. sapiens (human)
• Insert Size (bp): 10000
• Entrez Gene: ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
• Promoter: truncated mouse MT promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sst II (not destroyed)
• 3′ cloning site: Sst II (not destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13-rev(?)

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The DNA construct was made by Guido Jenster and Nora Heisterkamp in several cloning and subcloning steps. The transgenic construct contains human BCR exon 1, intron sequences of human BCR and ABL genes, human ABL exon 2 and ABL cDNA sequences. The transgene expression is controlled by a 200-bp Sst I- Bgl II fragment of the murine metallothionein-1 (Mt1) promoter lacking glucocorticoid-responsive elements (PMID 8541551). This BCR/ABL fusion gene produces a P190 protein and is a model for Philadelphia chromosome-positive acute lymphoblastic leukemia (ALL) in humans, which is caused by a chromosomal translocation that fuses the ABL proto-oncogene with breakpoint cluster region (BCR) sequences. Leukemia development is relatively rapid on inbred C57Bl/6J and CBA backgrounds and is slower on mixed genetic backgrounds. Mice transgenic for this construct on an inbred C57Bl/6J background are also available from Jackson labs stock 017833. The insert can be separated from the vector by digestion with Sst II.