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Abr DH domain pGEX
(Plasmid #38181)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 3X
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5837
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Rosetta
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABR DH domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    837
  • Mutation
    ABR DH domain
  • GenBank ID
    NM_001083.2
  • Entrez Gene
    ABR (a.k.a. MDB)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GST-F
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Vesa Kaartinen.
4-way ligation 0.19 kb Bgl II-BstEII + 0.4 kb BstEII-SstI + 0.2 kb SstI-EcoRI (EcoRI site is polylinker-derived) into pGEX3x BamHI x EcoRI.
N-terminus start: SEEIY (contains Bgl II) to KIKISQ (contains Bgl II) C-terminal. This corresponds approximately to nucleotides 232-1069 in NM_001083.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Abr DH domain pGEX was a gift from Nora Heisterkamp (Addgene plasmid # 38181 ; http://n2t.net/addgene:38181 ; RRID:Addgene_38181)

  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687
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