Skip to main content
Addgene

Bcr DH domain NE/AA pGEX
(Plasmid #38180)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX 3X
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5850
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Rosetta
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCR DH domain N689A/E690A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    850
  • Mutation
    BCR DH domain N689A/E690A
  • GenBank ID
    NM_004327
  • Entrez Gene
    BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GST-F
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Young Jin Cho.
Mutagenesis done using a Stratagene Quick Change kit.
The parent plasmid Bcr DH domain in pGEX 3X (AddGene #38179) was digested with SstIxPshAI and a 430 bp fragment containing the wt sequence was removed. This fragment was replaced by a fragment containing the NE/AA mutations from ECFP-Bcr N689A/E690A (AddGene #36418). The insert includes nucleotides 2118-2965 of BCR NM_004327.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bcr DH domain NE/AA pGEX was a gift from Nora Heisterkamp (Addgene plasmid # 38180 ; http://n2t.net/addgene:38180 ; RRID:Addgene_38180)

  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687
Related items:
Commonly requested with: