Bcr DH domain pGEX
(Plasmid #38179)

• Depositing Lab: Nora Heisterkamp
• Publication: Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.

Backbone

• Vector backbone: pGEX3X
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5850
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Rosetta
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BCR DH domain
• Species: H. sapiens (human)
• Insert Size (bp): 850
• Mutation: BCR DH domain
• GenBank ID: NM_004327
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GST-F
• 3′ sequencing primer: pGEX3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Vesa Kaartinen.
An 0.8 kb NheI x Bgl II fragment (BCR NM_004327 nucleotides 2118-2965) was blunted and subcloned into pSK digested with SmaI. Clones with a 5’ BamHI and 3’ EcoRI site were identified. The 566 bp fragment was inserted as a BamHI x EcoRI fragment into pGEX 3X.