pCDNA3.1Xpress-AbrGAP
(Plasmid #38177)

• Depositing Lab: Nora Heisterkamp
• Publication: Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 5800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Abr GAP domain
• Species: H. sapiens (human)
• Insert Size (bp): 800
• Mutation: ABR GAP domain including amino acids 597-859
• Entrez Gene: ABR (a.k.a. MDB)
• Tag / Fusion Protein:
  • Xpress (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Eco RI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Young Jin Cho.
An 0.8 kb BamHI xEcoRI fragment from AbrGAP in pGEX3x (AddGene #36407) was inserted into pcDNA BamHI xEcoRI. The insert starts at a natural PvuII site in the ABR sequence. The fragment contains the natural TAG and 3’ UT sequences and encodes amino acid residues 597-859 (Abr isoform B).