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Addgene

Fer in pAcG2T
(Plasmid #38167)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38167 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcG2T
  • Backbone manufacturer
    Pharmingen
  • Backbone size w/o insert (bp) 8530
  • Total vector size (bp) 11000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FER
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Entrez Gene
    FER (a.k.a. PPP1R74, TYK3, p94-Fer)
  • Promoter polyhedron
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GST-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Leena Haataja. 4-way ligation of BamHI/NsiI + NsiI/KpnI + KpnI/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Fer protein but not from amplified virus stocks. Large-scale yield of protein is relatively low.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fer in pAcG2T was a gift from Nora Heisterkamp (Addgene plasmid # 38167 ; http://n2t.net/addgene:38167 ; RRID:Addgene_38167)
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