Abr in pAcG2T
(Plasmid
#38162)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 38162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcG2T
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Backbone manufacturerPharmingen
- Backbone size w/o insert (bp) 8530
- Total vector size (bp) 11130
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameABR
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SpeciesH. sapiens (human)
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Insert Size (bp)2600
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GenBank IDNM_001092
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Entrez GeneABR (a.k.a. MDB)
- Promoter polyhedron
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GST-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by Leena Haataja. 4-way ligation of BamHI/StuI + StuI/BstEII + BstEII/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Abr protein.
Addgene sequencing found an E24G point mutation--this does not alter plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Abr in pAcG2T was a gift from Nora Heisterkamp (Addgene plasmid # 38162 ; http://n2t.net/addgene:38162 ; RRID:Addgene_38162)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/40/68/2f82199e-c160-11e1-bbd8-003048dd6500.pdf.940x940_q85_autocrop.png)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/70/15/2f89e21e-c160-11e1-bbd8-003048dd6500.pdf.940x940_q85_autocrop.png)