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Addgene

Abr-pLEF
(Plasmid #38157)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEF
  • Backbone size w/o insert (bp) 7400
  • Total vector size (bp) 10200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2800
  • GenBank ID
    NM_001092
  • Entrez Gene
    ABR (a.k.a. MDB)
  • Promoter SV40
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GST or T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.4 kb BamHI/BstEII + 3' 2.2 kb BstEII/EcoRI ABR fragments into pLEF digested with BamHIxEcoRI. The ABR insert contains the natural TAG and 3' UT sequences.

Addgene sequencing found an E24G point mutation--this does not alter plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Abr-pLEF was a gift from Nora Heisterkamp (Addgene plasmid # 38157 ; http://n2t.net/addgene:38157 ; RRID:Addgene_38157)

  • For your References section:

    Activity of the Bcr GTPase-activating domain is regulated through direct protein/protein interaction with the Rho guanine nucleotide dissociation inhibitor. Kweon SM, Cho YJ, Minoo P, Groffen J, Heisterkamp N. J Biol Chem. 2008 Feb 8;283(6):3023-30. Epub 2007 Dec 10. 10.1074/jbc.M705513200 PubMed 18070886
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