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EYFP-Abr in pCCL-cppt178-MNDU3
(Plasmid #38155)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCCL-cppt178-MNDU3
  • Backbone manufacturer
    Don Kohn [email protected]
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 9900
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Abr
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_021962
  • Entrez Gene
    ABR (a.k.a. MDB)
  • Promoter MNDU3 = LTR promoter for U3 region of myeloproliferative sarcoma retrovirus MND
  • Tag / Fusion Protein
    • EYFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age I (destroyed during cloning)
  • 3′ cloning site Kpn I (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Jess Cunnick. Abr was first subcloned into pEYFP-C1 Bgl II x KpnI as a 0.4 kb 5' BamHI/BstEII + 2.2 kb BstEII- KpnI fragment. The EYFP-Abr fusion was isolated as a 5' AgeI- 3' KpnI fragment and inserted into pCCL-cppt178-MNDU3-X3 digested with Xma I x Kpn I.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EYFP-Abr in pCCL-cppt178-MNDU3 was a gift from Nora Heisterkamp (Addgene plasmid # 38155 ; http://n2t.net/addgene:38155 ; RRID:Addgene_38155)

  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687
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