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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 38153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCCL-c-MNDU3c-(X2)-Pgk-EGFP
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Backbone manufacturerDon Kohn [email protected]
- Backbone size w/o insert (bp) 7900
- Total vector size (bp) 12000
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameN-cadherin
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SpeciesH. sapiens (human)
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Insert Size (bp)4100
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GenBank IDNM_001792
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Entrez GeneCDH2 (a.k.a. ACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD)
- Promoter MNDU3c = LTR promoter for U3 region of myeloproliferative sarcoma retrovirus MND
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer MSCV_rev
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameEGFP
- Promoter PGK
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer mPGK-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypurchased clone from Origene human N-cadherin in pCMV6-XL6
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by Bin Zhang. Vector was digested with EcoRI and the site blunted by filling in with T4 DNA polymerase. N-cadherin was isolated as a blunt-end fragment by digesting N-cadherin in pCMV6-XL with BsrBI (in front of the ATG) and with SmaI (in the vector). Has good N-cadherin expression. This vector expresses EGFP from the PGK promoter and non-tagged human N-cadherin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
N-cadherin in pCCL-c-MNDU3c-PGK-EGFP was a gift from Nora Heisterkamp (Addgene plasmid # 38153 ; http://n2t.net/addgene:38153 ; RRID:Addgene_38153) -
For your References section:
Increased resistance to a farnesyltransferase inhibitor by N-cadherin expression in Bcr/Abl-P190 lymphoblastic leukemia cells. Zhang B, Groffen J, Heisterkamp N. Leukemia. 2007 Jun;21(6):1189-97. Epub 2007 Mar 29. 10.1038/sj.leu.2404667 PubMed 17392819