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Addgene

pBA2219
(Plasmid #38152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    T7 promoter cassette and Gateway cloning cassette inserted into p15A replicon.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DsbC
  • Species
    E. coli
  • GenBank ID
    388476123
  • Entrez Gene
    dsbC (a.k.a. b2893, ECK2888, xprA)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal leader peptide (cloning artifact) (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA2219 was a gift from David Waugh (Addgene plasmid # 38152 ; http://n2t.net/addgene:38152 ; RRID:Addgene_38152)

  • For your References section:

    Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3). Austin BP, Waugh DS. Protein Expr Purif. 2012 Mar;82(1):116-24. Epub 2011 Dec 14. 10.1016/j.pep.2011.11.015 PubMed 22197595
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