Skip to main content
Addgene

pBA2257
(Plasmid #38151)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMal-C2
  • Backbone manufacturer
    New England Biolabls
  • Modifications to backbone
    Inserted Gateway Cloning Cassette in pMalC2 to create pKM596. Then inserted proMeCPA ORF by Gateway cloning.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    use BL21(DE3) for expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MBP-proMeCPA fusion protein
  • Species
    M. anisopliae
  • Promoter tac
  • Tag / Fusion Protein
    • maltose binding protein (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13_pUC_rev_primer
  • 3′ sequencing primer M13_pUC_fwd_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA2257 was a gift from David Waugh (Addgene plasmid # 38151 ; http://n2t.net/addgene:38151 ; RRID:Addgene_38151)

  • For your References section:

    Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3). Austin BP, Waugh DS. Protein Expr Purif. 2012 Mar;82(1):116-24. Epub 2011 Dec 14. 10.1016/j.pep.2011.11.015 PubMed 22197595
Related items:
Commonly requested with: