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Addgene

p1177-X1-8
(Plasmid #38150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac-1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6526
  • Total vector size (bp) 6522
  • Modifications to backbone
    Gateway cloning cassette was inserted into pFastBac-1 to create pDEST-8.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Carboxypeptidase A
  • Alt name
    MeCPA
  • Species
    M. anisopliae
  • Insert Size (bp)
    1278
  • GenBank ID
    EU919684
  • Promoter polyhedron
  • Tag / Fusion Protein
    • HHHHHHRR (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer polyhedron_fwd_primer
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1177-X1-8 was a gift from David Waugh (Addgene plasmid # 38150 ; http://n2t.net/addgene:38150 ; RRID:Addgene_38150)

  • For your References section:

    The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: insights into their use as tools for the removal of affinity tags. Austin BP, Tozser J, Bagossi P, Tropea JE, Waugh DS. Protein Expr Purif. 2011 May;77(1):53-61. Epub 2010 Nov 10. 10.1016/j.pep.2010.11.005 PubMed 21073956
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