pPK605
(Plasmid #38148)

• Depositing Lab: Patricia Kuwabara
• Publication: Kuwabara unpublished plasmids (unpublished)

Backbone

• Vector backbone: n/a
• Total vector size (bp): 15293
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR)
• Alt name: unc-119
• Species: C. elegans (nematode)
• Entrez Gene: unc-119 (a.k.a. CELE_M142.1)
• Promoter: unc-119

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13pUC-fwd

Gene/Insert 2

• Gene/Insert name: pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
• Species: C. elegans (nematode)
• Promoter: pie-1 promoter

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13pUC-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was derived from pID3.01B (Geraldine Seydoux - Addgene plasmid 22720)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid pPK605 contains two inserts: (1) unc-119 promoter, unc-119 and unc-119 3'UTR cassette and (2) pie-1 promoter, MluI/BamHI cloning site and pie-1 3'UTR

Please see plasmid map link above for more details