Skip to main content
Addgene

pPK720
(Plasmid #38147)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript KSII(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 14610
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dpy-7p::ptc-3::gfp
  • Alt name
    dpy-7 promoter
  • Alt name
    ptc-3
  • Entrez Gene
    ptc-3 (a.k.a. Y110A2AL.8)
  • Promoter dpy-7
  • Tag / Fusion Protein
    • EGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13pUC-rev
  • 3′ sequencing primer M13pUC-fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pPK700 plasmid was derived by ligating the dpy-7 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPK720 was a gift from Patricia Kuwabara (Addgene plasmid # 38147 ; http://n2t.net/addgene:38147 ; RRID:Addgene_38147)

  • For your References section:

    C. elegans patched-3 is an essential gene implicated in osmoregulation and requiring an intact permease transporter domain. Soloviev A, Gallagher J, Marnef A, Kuwabara PE. Dev Biol. 2011 Mar 15;351(2):242-53. Epub 2011 Jan 4. 10.1016/j.ydbio.2010.12.035 PubMed 21215260
Related items:
Commonly requested with: