pPJ-1
(Plasmid #38144)

• Depositing Lab: Patricia Kuwabara
• Publication: Joyce et al PLoS Genet. 2012 Mar;8(3):e1002602. Epub 2012 Mar 29.

Backbone

• Vector backbone: pPD122.56
• Backbone manufacturer: Andrew Fire (Addgene plasmid# 1632)
• Total vector size (bp): 4464
• Modifications to backbone: pPJ-1 was generated by excising the GFP(S65C) fragment from vector pPD112.56 and replacing it with an mRFP sequence amplified with primers PK504 and PK505 using the pmRFP_C1 vector.
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mRFP
• Insert Size (bp): 697
• Promoter: promoterless

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13pUC-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Promoterless host vector allows any promoter to be cloned upstream of SV40NLS::mRFP cassette for use as a transcriptional expression reporter.