pC-GoldyTALEN
(Plasmid #38143)

• Purpose: destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter
• Depositing Lab: Daniel Carlson
• Publication: Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Backbone

• Vector backbone: pBSK
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5702
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GoldyTALEN
• Alt name: TALEN
• Species: Synthetic
• Insert Size (bp): 1800
• Mutation: Truncate at N and C terminous
• Promoter: miniCaggs
• Tags / Fusion Proteins:
  • FokI homodimer (C terminal on insert)
  • AcV5 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Esp31 (destroyed during cloning)
• 3′ cloning site: Esp31 (destroyed during cloning)
• 5′ sequencing primer: CGTGACCGCAATGGAGGC
• 3′ sequencing primer: CACTGCATCCATGGCAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A derivative of pTAL-Leu, a TALEN plasmid submitted by the Dan Voytas lab.
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899).

For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson