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Addgene

RCIscript-GoldyTALEN Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: SacI single digest and SacI+StuI double digest.

We expected the following band sizes for this digest: 4.6kb band for SacI and 1.6kb+3kb bands for SacI/StuI double. We see the expected pattern.