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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Tag 2b
- Backbone size w/o insert (bp) 4300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNLRP10
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SpeciesH. sapiens (human)
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Insert Size (bp)1900
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Entrez GeneNLRP10 (a.k.a. CLR11.1, NALP10, NOD8, PAN5, PYNOD)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer t3
- 3′ sequencing primer t7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-NLRP10 was a gift from Thomas Kufer (Addgene plasmid # 38141 ; http://n2t.net/addgene:38141 ; RRID:Addgene_38141) -
For your References section:
NLRP10 enhances Shigella-induced pro-inflammatory responses. Lautz K, Damm A, Menning M, Wenger J, Adam AC, Zigrino P, Kremmer E, Kufer TA. Cell Microbiol. 2012 Jun 4. doi: 10.1111/j.1462-5822.2012.01822.x. 10.1111/j.1462-5822.2012.01822.x PubMed 22672233