Skip to main content
Addgene

pcDNA4-FLAG-Jhdm2a
(Plasmid #38136)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA4/myc-His A
  • Backbone size w/o insert (bp) 5100
  • Total vector size (bp) 9100
  • Modifications to backbone
    "AAGCTTATGGACTACAAAGACGATGACGACAAGGGATCC" FLAG linker is inserted to HindIII/BamHI site.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jhdm2a
  • Alt name
    Kdm3a
  • Alt name
    jmjd1a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4000
  • Mutation
    protein starts at aa115
  • Entrez Gene
    Kdm3a (a.k.a. 1700105C21Rik, C230043E16Rik, JHDM2a, Jmjd1, Jmjd1a, KDM2A, TGSA, Tsga)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-FLAG-Jhdm2a was a gift from Toshinobu Nakamura & Toru Nakano (Addgene plasmid # 38136 ; http://n2t.net/addgene:38136 ; RRID:Addgene_38136)

  • For your References section:

    PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos. Nakamura T, Liu YJ, Nakashima H, Umehara H, Inoue K, Matoba S, Tachibana M, Ogura A, Shinkai Y, Nakano T. Nature. 2012 Jun 3;486(7403):415-9. doi: 10.1038/nature11093. 10.1038/nature11093 PubMed 22722204
Related items:
Commonly requested with: