pGE-mSmo-YFP
(Plasmid
#38094)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38094 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGE
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Modifications to backboneEGFP removed from pEGFP-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmo
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SpeciesM. musculus (mouse)
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Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
- Promoter CMV
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGE-mSmo-YFP was a gift from Philip Beachy (Addgene plasmid # 38094 ; http://n2t.net/addgene:38094 ; RRID:Addgene_38094) -
For your References section:
Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened. Chen JK, Taipale J, Cooper MK, Beachy PA. Genes Dev. 2002 Nov 1. 16(21):2743-8. 10.1101/gad.1025302 PubMed 12414725