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Addgene

pFRT/TO/HIS/FLAG/HA-PRDX1
(Plasmid #38086)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38086 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFRT/TO/HIS/FLAG/HA-DEST
  • Backbone size w/o insert (bp) 5321
  • Total vector size (bp) 5921
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRDX1 peroxiredoxin 1
  • Alt name
    PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • GenBank ID
    NM_002574.3
  • Entrez Gene
    PRDX1 (a.k.a. MSP23, NKEF-A, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2)
  • Tag / Fusion Protein
    • HIS/FLAG/HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/TO/HIS/FLAG/HA-PRDX1 was a gift from Markus Landthaler (Addgene plasmid # 38086 ; http://n2t.net/addgene:38086 ; RRID:Addgene_38086)
  • For your References section:

    The mRNA-Bound Proteome and Its Global Occupancy Profile on Protein-Coding Transcripts. Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M. Mol Cell. 2012 Jun 8;46(5):674-90. 10.1016/j.molcel.2012.05.021 PubMed 22681889