pFRT/TO/HIS/FLAG/HA-KIAA1967
(Plasmid
#38082)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 38082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFRT/TO/HIS/FLAG/HA-DEST
- Backbone size w/o insert (bp) 5539
- Total vector size (bp) 8311
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKIAA1967
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Alt nameDBC1; DBC-1; NET35; p30DBC; p30 DBC
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SpeciesH. sapiens (human)
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Insert Size (bp)2772
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GenBank IDNM_021174.5
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Entrez GeneCCAR2 (a.k.a. DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC)
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Tag
/ Fusion Protein
- HIS/FLAG/HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT/TO/HIS/FLAG/HA-KIAA1967 was a gift from Markus Landthaler (Addgene plasmid # 38082 ; http://n2t.net/addgene:38082 ; RRID:Addgene_38082) -
For your References section:
The mRNA-Bound Proteome and Its Global Occupancy Profile on Protein-Coding Transcripts. Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M. Mol Cell. 2012 Jun 8;46(5):674-90. 10.1016/j.molcel.2012.05.021 PubMed 22681889