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pFRT/TO/HIS/FLAG/HA-API5
(Plasmid #38074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT/TO/HIS/FLAG/HA-DEST
  • Backbone size w/o insert (bp) 5496
  • Total vector size (bp) 7011
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    API5 apoptosis inhibitor 5
  • Alt name
    AAC11; AAC-11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1515
  • GenBank ID
    NM_006595.3
  • Entrez Gene
    API5 (a.k.a. AAC-11, AAC11)
  • Tag / Fusion Protein
    • HIS/FLAG/HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/TO/HIS/FLAG/HA-API5 was a gift from Markus Landthaler (Addgene plasmid # 38074 ; http://n2t.net/addgene:38074 ; RRID:Addgene_38074)

  • For your References section:

    The mRNA-Bound Proteome and Its Global Occupancy Profile on Protein-Coding Transcripts. Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M. Mol Cell. 2012 Jun 8;46(5):674-90. 10.1016/j.molcel.2012.05.021 PubMed 22681889
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