pFRT/TO/HIS/FLAG/HA-PGK1
(Plasmid #38071)

• Depositing Lab: Markus Landthaler
• Publication: Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Backbone

• Vector backbone: pFRT/TO/HIS/FLAG/HA-DEST
• Backbone size w/o insert (bp): 5511
• Total vector size (bp): 6765
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PGK1 phosphoglycerate kinase 1
• Alt name: PGKA; MIG10
• Species: H. sapiens (human)
• Insert Size (bp): 1254
• GenBank ID: NM_000291.3
• Entrez Gene: PGK1 (a.k.a. HEL-S-68p, MIG10, PGKA)
• Tag / Fusion Protein:
  • HIS/FLAG/HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry