pFRT/TO/HIS/FLAG/HA-HNRNPU
(Plasmid #38068)

• Depositing Lab: Markus Landthaler
• Publication: Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Backbone

• Vector backbone: pFRT/TO/HIS/FLAG/HA-DEST
• Backbone size w/o insert (bp): 5496
• Total vector size (bp): 7917
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• Alt name: HNRPU; SAF-A; U21.1; hnRNP U
• Species: H. sapiens (human)
• Insert Size (bp): 2421
• GenBank ID: NM_004501.3
• Entrez Gene: HNRNPU (a.k.a. DEE54, EIEE54, GRIP120, HNRNPU-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp120)
• Tag / Fusion Protein:
  • HIS/FLAG/HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry