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Addgene

pXCX.CMV.CREB.WPRE
(Plasmid #38050)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38050 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXCXCMV
  • Total vector size (bp) 10960
  • Modifications to backbone
    co-expresses EGFP using an IRES
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CREB
  • Alt name
    cAMP responsive element binding protein 1
  • Alt name
    creb1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1026
  • GenBank ID
    NM_031017.1
  • Entrez Gene
    Creb1 (a.k.a. Creb)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Adenoviral plasmid for overexpression of CREB from CMV promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXCX.CMV.CREB.WPRE was a gift from James Uney (Addgene plasmid # 38050 ; http://n2t.net/addgene:38050 ; RRID:Addgene_38050)

  • For your References section:

    Adenoviral expression of CREB protects neurons from apoptotic and excitotoxic stress. Glover CP, Heywood DJ, Bienemann AS, Deuschle U, Kew JN, Uney JB. Neuroreport. 2004 May 19;15(7):1171-5. 10.1097/00001756-200405190-00018 PubMed 15129168
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