pAAV-EF1a-FLEX-TVA-mCherry
(Plasmid #38044)

• Depositing Lab: Naoshige Uchida
• Publication: Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5604
• Total vector size (bp): 6780
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Avian sarcoma and leukemia virus receptor 950
• Alt name: TVA950
• Species: Synthetic; quail
• Insert Size (bp): 1145
• GenBank ID: AY531260.1
• Entrez Gene: CD320 (a.k.a. TVA)
• Promoter: EF1a
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: AscI (destroyed during cloning)
• 5′ sequencing primer: EF1a-forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: John A. T. Young
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry