Skip to main content
Addgene

pCS2+ABCB1a
(Plasmid #38036)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4851
  • Vector type
    Sea urchin, zebrafish, Xenopus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABCB1a
  • Alt name
    P-gp
  • Alt name
    MDR1
  • Species
    Strongylocentrotus purpuratus
  • Insert Size (bp)
    3984
  • GenBank ID
    JQ390048
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CTTGATTTAGGTGACATATAG
  • 3′ sequencing primer TGTTGTTAACTTGTTTATTGCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ABCB1a was a gift from Amro Hamdoun (Addgene plasmid # 38036 ; http://n2t.net/addgene:38036 ; RRID:Addgene_38036)

  • For your References section:

    Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR) Efflux Transporters. Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A. J Biol Chem. 2012 Nov 2. 10.1074/jbc.M112.424879 PubMed 23124201
Related items: