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pLVTHM-shREV3-4
(Plasmid #38033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVTHM
  • Backbone manufacturer
    Trono Laboratory
  • Backbone size w/o insert (bp) 11085
  • Total vector size (bp) 12000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shREV3-4
  • Alt name
    REV3L
  • gRNA/shRNA sequence
    CAAAGATGCTGCTACATTA
  • Species
    H. sapiens (human), M. musculus (mouse), R. norvegicus (rat); human
  • GenBank ID
    1.NM_002912.3
  • Entrez Gene
    Rev3l (a.k.a. R, Rev, Rev3, Sez, Sez4)
  • Entrez Gene
    Rev3l
  • Entrez Gene
    REV3L (a.k.a. POLZ, REV3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer ?
  • 3′ sequencing primer ?
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A versatile tool for conditional gene expression and knockdown.

Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P.

Nat Methods. 2006 Feb;3(2):109-16.

PMID:16432520

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVTHM-shREV3-4 was a gift from Emanuela Felley-Bosco & Rolf Stahel (Addgene plasmid # 38033 ; http://n2t.net/addgene:38033 ; RRID:Addgene_38033)

  • For your References section:

    Inhibition of REV3 expression induces persistent DNA damage and growth arrest in cancer cells. Knobel PA, Kotov IN, Felley-Bosco E, Stahel RA, Marti TM. Neoplasia. 2011 Oct;13(10):961-70. PubMed 22028621
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