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pSUPERIOR.puro-shREV3-5
(Plasmid #38030)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPERIOR.puro
  • Backbone manufacturer
    oligoengine
  • Backbone size w/o insert (bp) 4354
  • Total vector size (bp) 4400
  • Vector type
    RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shREV3-5
  • gRNA/shRNA sequence
    GATATTCCATCTGTTACAA
  • Species
    H. sapiens (human), R. norvegicus (rat); human
  • GenBank ID
    1.NM_002912.3
  • Entrez Gene
    REV3L (a.k.a. POLZ, REV3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13
  • 3′ sequencing primer M13r
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPERIOR.puro-shREV3-5 was a gift from Emanuela Felley-Bosco & Rolf Stahel (Addgene plasmid # 38030 ; http://n2t.net/addgene:38030 ; RRID:Addgene_38030)

  • For your References section:

    Inhibition of REV3 expression induces persistent DNA damage and growth arrest in cancer cells. Knobel PA, Kotov IN, Felley-Bosco E, Stahel RA, Marti TM. Neoplasia. 2011 Oct;13(10):961-70. PubMed 22028621
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