ptdEos-CLC
(Plasmid #38009)

• Depositing Lab: Xiaowei Zhuang
• Publication: Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6028
• Modifications to backbone: EYFP coding sequence removed with NheI (5') and EcoRI (3').
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Clathrin, light polypeptide (LCa)
• Alt name: Clta
• Species: M. musculus (mouse)
• Insert Size (bp): 657
• GenBank ID: NM_001080385
• Entrez Gene: Clta (a.k.a. Lca)
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: CMV primer

Gene/Insert 2

• Gene/Insert name: Eos FP, tandem dimer
• Alt name: tdEos
• Insert Size (bp): 1348
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: tdEos was derived from tdEos-Vinculin, a kind gift of Dr. Michael W. Davidson (Florida State University). Clathrin light chain was a kind of gift of Dr. James H. Keen (Kimmel Cancer Center).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function. It is also missing its stop codon, so the peptide "PGPGSTGSR*" from the backbone is appended to the C-terminus of the CLC. The affect of this peptide on protein function is unknown.