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Addgene

pFastBac1/MBP-His
(Plasmid #38008)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 38008 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    FastBac-Dual
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Insect Expression ; Baculovirus expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBP (Maltose binding protein)
  • Species
    Synthetic
  • Insert Size (bp)
    1184
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6xHIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer polyhedrin-fwd
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1/MBP-His was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 38008 ; http://n2t.net/addgene:38008 ; RRID:Addgene_38008)
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