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pCMV-Neo-Bam E2F3a
(Plasmid #37970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37970 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 8000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E2F3a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1450
  • Entrez Gene
    E2F3 (a.k.a. E2F-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.

The 5' end of the coding sequence can be difficult to sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Neo-Bam E2F3a was a gift from Jacqueline Lees (Addgene plasmid # 37970 ; http://n2t.net/addgene:37970 ; RRID:Addgene_37970)

  • For your References section:

    The retinoblastoma protein binds to a family of E2F transcription factors. Lees JA, Saito M, Vidal M, Valentine M, Look T, Harlow E, Dyson N, Helin K. Mol Cell Biol. 1993 Dec;13(12):7813-25. PubMed 8246996
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