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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-Neo-Bam
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Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 8000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDP1
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Alt nameDRTF1
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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Entrez GeneTFDP1 (a.k.a. DILC, DP1, DRTF1, Dp-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer Bglob-intron-F
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHelin K, Ed Harlow's laboratory where the principal investigator (J.A.Lees) was working.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DP1 functions as a heterodimer with several members of the E2F family of transcription factors.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Neo-Bam DP1 was a gift from Jacqueline Lees (Addgene plasmid # 37968 ; http://n2t.net/addgene:37968 ; RRID:Addgene_37968) -
For your References section:
E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry. Moberg K, Starz MA, Lees JA. Mol Cell Biol. 1996 Apr;16(4):1436-49. PubMed 8657117