pCMV-Neo-Bam E2F4
(Plasmid #37966)

• Depositing Lab: Jacqueline Lees
• Publication: Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

Backbone

• Vector backbone: pCMV-Neo-Bam
• Backbone manufacturer: Vogelstein Lab
• Backbone size w/o insert (bp): 6600
• Total vector size (bp): 8000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E2F4
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: E2F4 (a.k.a. E2F-4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Bglob-intron-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone vector is Addgene #16440

E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.