Skip to main content
Addgene

pLKO.1 EB1 shRNA #3
(Plasmid #37927)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Addgene plasmid 8453
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EB1 shRNA #3
  • gRNA/shRNA sequence
    ATAATTCCTGTGGACAAAT
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAPRE1 (a.k.a. EB1)
  • Promoter human U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1-5
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 EB1 shRNA #3 was a gift from Torsten Wittmann (Addgene plasmid # 37927 ; http://n2t.net/addgene:37927 ; RRID:Addgene_37927)

  • For your References section:

    EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Gierke S, Wittmann T. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. 10.1016/j.cub.2012.02.069 PubMed 22483942
Related items:
Commonly requested with: